Bio-Rad Rotofor® and Mini Rotofor Cells - Manual (Page 6)

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Step 1: Assemble the Anode and Cathode Electrode Chambers
1. Place one grey gasket over the two alignment pins of the inner component
of the anode electrode. The three oblong holes in the gaskets should not
obstruct the six holes of the electrolyte chamber.
2. Remove the cation exchange membrane equilibrated in the acidic electrolyte
solution. DO NOT DISCARD THE BUFFER. Rinse the membrane with
water to remove residual acid.
3. Place the cation exchange membrane on the grey gasket by aligning the
notches in the membrane with the pins of the inner electrode compartment.
Cover the membrane with another grey gasket. Make sure that there is a
small O-ring inset in the central shaft of the outer portion of the electrode
assembly and fasten the halves of the electrode assembly together by finger
tightening the captive, threaded sleeve.
Fig. 2. The “Ion exchange membrane and silicone gasket sandwich” assembled
on the inner component of electrode assembly.
4. Repeat the assembly process for the cathode electrode.
5. Fill each electrode chamber with electrolyte immediately after assembly to
prevent the membranes from drying. Add 33 ml of the appropriate electrolyte
to each chamber and replace the vent buttons. The acidic electrolyte goes
into the anode electrode (red dot) and basic electrolyte goes into the cath-
ode electrode (black dot).
LIT603B 7/10/98 11:56 AM Page 4