Bio-Rad Rotofor® and Mini Rotofor Cells - Manual (Page 7)

Step 2: Assemble the Focusing Chamber
1. Slide the assembled anode electrode assembly over the ceramic cooling fin-
ger so that the two protruding screw heads fit into the holes in the black
plastic base of the cooling finger support assembly.
Fig. 3. Anode electrode assembled on the cooling finger.
2. Slide the membrane core onto the ceramic cooling finger, making sure the
core abuts the acrylic ridge on the anode chamber.
3. Slide the focusing chamber over the membrane core, inserting the metal
pin into the small hole in the anode chamber. Remove one of the black
port covers to visually check the position the focusing chamber. Each mem-
brane screen should lie between two adjacent ports. These ports must not be
blocked by the membrane screens. If the ports are blocked, remove the
focusing chamber, and slide it once more over the membrane core. Tighten
the black, nylon retaining screws. Check again to make sure the membrane
screens do not block the ports of the chamber.
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