Bio-Rad Rotofor® and Mini Rotofor Cells - Manual (Page 9)

5. Mount the assembled focusing chamber in the stand. The gear on the cath-
ode electrode assembly should be fully engaged with the gear on the base
unit. If the focusing chamber does not slide in easily, remove it to check
that all parts are properly assembled.
6. Attach the power cord to the back of the unit and connect it to an electrical
outlet.
Step 3: Prepare the Focusing Chamber
1. With the cell mounted on the stand, rotate the focusing chamber so the col-
lection ports, identified by the two metal alignment pins, are facing up.
2. Cover the ports with a piece of the sealing tape (10.5 cm) provided with
the cell.
3. Reinforce the taped ports with one of the two black acrylic cell-cover blocks,
and finger tighten the screws.
Step 4: Load the Sample
1. Turn the toggle switches to ON and HARVEST. In the harvest mode the
focusing chamber will automatically stop with the filling ports facing up
and the collection ports facing down.
2. Prepare the sample:
A. For the standard focusing chamber combine the following in a beaker:
58 ml dH
2
O
1 ml sample
3 ml Bio-Lyte ampholytes (pH range 3/10)
B. For the mini focusing chamber combine the following in a beaker:
18.5 ml dH
2
O
0.5 ml sample
1.0 ml Bio-Lyte ampholytes (pH range 3/10)
3. Fill the cell with sample through the loading ports using the syringe provided
in this kit. Typically, every other port is filled, and the sample spreads into
the adjoining compartments.
For the standard focusing chamber, the minimum sample volume must be
sufficient to cover the cooling finger (33 ml minimum; 58 ml maximum). For
the mini focusing chamber always load the maximum sample volume of 18 ml.
7
LIT603B 7/10/98 11:57 AM Page 7