Operator’s manual – ANKOM 200 Series User Manual
Page 19
Operator’s Manual
Rev C 1/14/15
pg. 19
NDF Analysis Procedure using the ANKOM
200
Fiber Analyzer
To perform NDF analysis on prepared samples, follow the procedure detailed below.
The Vessel must be at room temperature before beginning an NDF analysis.
1. Verify that the Exhaust Hose is connected to the instrument and securely positioned in the drain.
2. Turn the instrument Power Switch to the ON position.
3. Lift the Lid Handle to open the Vessel Lid.
4. Place the Bag Suspender with the samples and
the Bag Suspender Weight into the Vessel.
5. Pour a maximum of 2 L of Neutral Detergent
(ND) solution into the Vessel along with 20 g
of sodium sulfite (0.5 g / 50 ml of ND
solution) and 4.0 ml of α-amylase.
6. Press the
H
H
E
E
A
A
T
T
and
A
A
G
G
I
I
T
T
A
A
T
T
E
E
buttons on the
front of the instrument so they are lit. Look in
the Vessel to confirm agitation.
7. Close the Vessel Lid.
8. On the Timer/Clock, set the Timer to 75
minutes and press the Start/Stop button.
9. When the Timer sounds, the extraction
process is complete. Press the
H
H
E
E
A
A
T
T
and
A
A
G
G
I
I
T
T
A
A
T
T
E
E
buttons to turn them off (the buttons
will not be lit when they are off). Open the
Exhaust Valve (slowly at first) and exhaust the
hot solution BEFORE opening the Vessel Lid.
Because the Vessel is under pressure, the Exhaust Valve must be opened to
release the pressure and the solution BEFORE opening the Vessel Lid.
10. After the solution has been exhausted, open the Vessel Lid slowly to allow air to push any remaining
liquid out the Exhaust Hose.
11. Close the Exhaust Valve.
12. Add 1900 ml - 2000 ml of 70°C - 90°C rinse water and 4.0 ml of α-amylase.
During the rinse process, if the
H
H
E
E
A
A
T
T
button is OFF, the Vessel Lid can be
open. If the
H
H
E
E
A
A
T
T
button is ON, the Vessel Lid must be closed.
13. Press the
A
A
G
G
I
I
T
T
A
A
T
T
E
E
button.
14. On the Timer/Clock, set the Timer to 5 minutes and press the Start/Stop button.
15. When the Timer sounds, the rinse is complete. Slowly open the Exhaust Valve to drain the hot water.
16. Repeat steps 12 - 16 to accomplish the second rinse.
17. Repeat steps 12 - 16 without adding α-amylase to accomplish the third rinse.
Agitate Button
Heat Button
Timer/Clock
Temperature
Controller
Power Switch
(out of view)
Exhaust Hose Connector
Exhaust Valve Handle
Lid Handle
Vessel Lid
Vessel