ANKOM 200 Series - Manual (Page 34)

Operator’s Manual
pg. 34
Rev C 1/14/15
Calculations
% NDF (as-received basis) = 100 x (W
3
– (W
1
x C
1
))
W
2
Where: W
1
= Bag tare weight
W
2
= Sample weight
W
3
= Dried weight of bag with fiber
after extraction process
C
1
= Blank bag correction (running
average of final oven-dried
weight divided by original blank
bag weight)
NDF Procedure (continued)
8. Place up to 3 bags on each of eight Bag Suspender Trays
(maximum of 24 bags). Stack the trays on the center post of
the Bag Suspender with each level rotated 120 degrees in
relation to the tray below it. Place the empty 9th tray on top.
NOTE: All nine trays must be used regardless of the number
of bags being processed.
9. Verify that the hot water supply is on and the drain hose is
securely positioned in the drain.
10. Turn the instrument Power Switch to the ON position.
11. Open the Vessel Lid and insert the Bag Suspender with bags
into the Vessel and place the Bag Suspender weight on top
of the empty 9
th tray to keep the Bag Suspender submerged.
12. When processing 24 sample bags, add 1900-2000 mL of
ambient temperature ND solution to the fiber analyzer
vessel. If processing less than 20 bags, add 100 mL/bag of
ND solution (use minimum of 1500 mL to ensure Bag
Suspender is covered). Add 20 g (0.5 g per 50 mL of ND
solution) of sodium sulfite and 4.0 mL of alpha-amylase to
the solution in the vessel.
13. Turn Agitate and Heat ON and confirm agitation.
14. Set the timer for 75 minutes and close the lid.
15. When the NDF extraction is complete, turn Agitate and Heat
OFF.
16. Open the drain valve (slowly at first) and exhaust the hot
solution before opening the Vessel Lid. NOTE: The solution
in the Vessel is under pressure. The exhaust valve needs to
be opened to release the pressure and solution prior to
opening the Vessel Lid.
17. After the solution has been exhausted, close the exhaust
valve and open the Vessel Lid. Add 1900-2000 mL of 70-
90°C rinse water and 4.0 mL of alpha-amylase to the first
and second rinses. Turn Agitate on and rinse for 5 minutes.
If the Heat is ON, the Vessel Lid should be closed. If the
Heat is OFF, the Vessel Lid can be open. Repeat 5 minute
hot water rinse 1 more time for a total of 3 rinses.
18. When the NDF extraction and rinsing procedures are
complete, open the Vessel Lid and remove the filter bags.
Gently press out excess water from the bags. Place bags in a
250ml beaker and add enough acetone to cover bags and
soak for 3-5 minutes.
19. Remove the filter bags from the acetone and place them on a
wire screen to air-dry. Completely dry in an oven at 102 ±
2°C. (In most ovens the filter bags will be completely dry
within 2-4 hours.) NOTE: Do not place bags in the oven
until the acetone in the bags has completely evaporated.
20. Remove the filter bags from the oven and immediately place
them directly into a collapsible desiccant pouch and flatten
to remove any air. Cool to ambient temperature and weigh
the filter bags (W
3
). NOTE: Do not use a conventional
countertop or cabinet desiccator.