Operator’s manual – ANKOM 200 Series User Manual
Page 24
Operator’s Manual
pg. 24
Rev C 1/14/15
16. When the Timer sounds, the rinse is complete. Slowly open the Exhaust Valve to drain the hot water.
17. Repeat steps 12 - 16 to accomplish the second rinse.
18. Add 1900 ml - 2000 ml of ambient temperature base (0.313N NaOH) solution.
19. Press the
H
H
E
E
A
A
T
T
and
A
A
G
G
I
I
T
T
A
A
T
T
E
E
buttons on the front of the instrument so they are lit. Look in the Vessel to
confirm agitation.
20. Close the Vessel Lid.
21. On the Timer/Clock, set the Timer to 40 minutes and press the Start/Stop button.
22. When the Timer sounds, the extraction is complete. Press the
H
H
E
E
A
A
T
T
and
A
A
G
G
I
I
T
T
A
A
T
T
E
E
buttons to turn them
off (the buttons will not be lit when they are off). Open the Exhaust Valve (slowly at first) and exhaust
the hot solution BEFORE opening the Vessel Lid.
Because the Vessel is under pressure, the Exhaust Valve must be opened to
release the pressure and the solution BEFORE opening the Vessel Lid.
23. After the solution has been exhausted, open the Vessel Lid slowly to allow air to push any remaining
liquid out the Exhaust Hose.
24. Close the Exhaust Valve.
25. Add 1900 ml - 2000 ml of 50°C - 90°C rinse water.
During the rinse process, if the
H
H
E
E
A
A
T
T
button is OFF, the Vessel Lid can be
open. If the
H
H
E
E
A
A
T
T
button is ON, the Vessel Lid must be closed.
26. Press the
A
A
G
G
I
I
T
T
A
A
T
T
E
E
button.
27. On the Timer/Clock, set the Timer to 5 minutes and press the Start/Stop button.
28. When the Timer sounds, the rinse is complete. Slowly open the Exhaust Valve to drain the hot water.
29. Repeat steps 24 - 28 two times (total of three rinses).
30. After the third rinse, open the Vessel Lid and remove the Bag Suspender from the Vessel.
31. Remove the bags from the Bag Suspender trays and place them in a 250 ml beaker.
32. With your hands, gently press out excess water from the bags into the
beaker and pour off the water from the beaker.
33. With the bags in a 250 ml beaker, add enough acetone to cover them. Let
the bags soak in acetone for 3 - 5 minutes. Pour off the acetone.
34. With your hands, gently press out excess acetone from the bags into the
beaker and pour off the acetone from the beaker.
35. Remove the bags from the beaker and place them on a wire screen to air-dry.
Hazardous Materials
–
Do NOT place bags in an oven until all acetone in
the bags has evaporated.
36. Place air-dried bags in the oven and heat at 102°C ± 2° for 2 - 4 hours (depending on the oven).