ANKOM HCl Series - Manual (Page 16)

Operator’s Manual
pg. 16 Rev L 11/12/14
Sample Preparation Procedure
To avoid fat migration from one filter bag to another during the hydrolysis procedure, it is very important to
prepare your samples properly.
Before running production samples, it is recommended that you do the
following:
 Categorize your samples to determine the standard amount of DE needed
for each of your specific sample types. See the “Sample Categorization”
section for details.
 Mix dry/granular samples with the correct amount of DE.
 Cover moist samples with the correct amount of DE.
To prepare samples for hydrolysis, follow the procedure detailed below.
1. Using a Solvent Resistant Marker, number all of the empty filter bags you will
use during the hydrolysis procedure.
2. Record the weights of all the filter bags prior to filling them.
3. Place an empty filter bag in the Bag Holder in an open position.
4. Tare the weight of the empty filter bag and the holder together.
5. Add the necessary DE and sample to the filter bag for Dry/Granular, Moist, and Liquid samples. (See the
“Sample Categorization” section for help in determining how much DE to add to the filter bag.)
For Dry/Granular or Moist samples:
The total weight of DE and sample dry matter must NOT exceed 1.5g. Too
much volume inside the bags will make it difficult for the acid to completely
saturate the sample during hydrolysis. This may reduce the number of bags
that can be processed in the Hydrolysis Vessel at one time.
For Liquid samples:
The total weight of DE and sample MAY exceed 1.5g.
Dry/Granular Samples
a. Add the full recommended amount of DE to the filter bag.
b. Record the weight of the DE and tare.
c. Add an appropriate amount of sample to the filter bag. Keep all
particles away from the sealing area of the filter bag.
d. Record the weight of the sample (W
1
).