ANKOM HCl Series - Manual (Page 18)

Operator’s Manual
pg. 18 Rev L 11/12/14
11. Include two blank bags (C
1
and C
2
) filled with approximately 0.5g – 0.75g of DE.
Blank bags serve as correction factors for slight losses of weight that may
occur during extraction. Blank bags with DE also act as indicators of fat loss
during hydrolysis. When placed next to a sample that loses fat, a blank bag
will absorb some of the fat lost by the other bag. The result is a blank loss
during extraction that exceeds the normal expected blank loss of 0.0025g by
0.0005g or more. This indicates that fat migrated from a bag with sample to
a blank bag during hydrolysis, and then that same fat was lost during
extraction. If fat migrates from the bags, a pre-extraction with solvent may
be necessary.
12. Insert filter bags into the Multi-bag Holder and snap the handle in place. Samples
are now ready for the hydrolysis procedure.