ChemoMetec NC-3000 - Manual (Page 14)

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Template Setup
Once compensation factors, quadrants, polygons, markers and gating options
have been defined for a sample, these settings can easily replicated to all
future analyses acquired with a specific protocol.
Check the box associated with the sub-population of interest and the de-
sired action such as ‘Include’ or ‘Exclude’.
Click ‘Apply’ to show the affect of the gating chosen and ‘OK’ to save. If
gating is not required, check the ‘Disable’ box followed by ‘Okay’.
Note: If multiple gates has been selected for inclusion or exclusion, they can be
combined by either Union or Intersection of the populations.
• Union: This option is available when two gates have been set to either
Include or Exclude.
Selecting this option results in display of the sum of all events dis
played for the two gates (included or excluded).
• Intersection: This option is available when two gates have been set to
either Include or Exclude.
Selecting this option results in display of the events displayed for both
of the two gates (included or excluded)
Double-click on the small graph to be analyzed to open the correspond-
ing larger graph in editing mode.
Click the ‘Gating Setup’ in the upper left corner to open a list of gating op-
tions available for that particular analysis. Gates options are denoted as
‘P’ for polygon, ‘Q’ for quadrant and ‘M’ for markers followed by a number
indicating the order of creation. The most commonly used functions listed
are to ‘Include’ or ‘Exclude’ sub-populations but more advanced setting
may also be listed.