ChemoMetec NC-3000 - Manual (Page 9)

8
Creating and Modifying Graphs
Default graphs representing each of the channels measured in a particular
analysis or graphs defined by the master in the protocol are automatically
opened in Plot Manager. However, these graphs may be modified for better
viewing by changing the axis scale or parameter or adding new scatter plots
may be required for data analysis.
Double-click on the appropriate icon on the left-hand side of the data row
representing either a scatter plot or a histogram .
A small plot with default settings will have been added to the correspond-
ing data row in Plot Manager. Double-click on the small plot to open it in
editing mode.
Axis scale and parameters can be changed and only those parameters
available for that particular analysis will be available from the drop down
menu. Axis scale can be returned to default setting by leaving fields blank
or entering the number in the gray fields immediately below.
Click ‘OK’ to save changes and close the large graph.
Figure 6 . A) Scatter plot showing GFP fluorescence in the green and or-
ange channel after compensation. Note, no increase in orange fluores-
cence is seen as green fluorescence increases. B) Compensation matrix
adjusted for 0.8% signal spillover from the GFP into the orange channel.
A)
B)