Hoefer IEF100 - Manual (Page 52)

• p46
Appendix A: Preprogrammed Protocols
The following 9 protocols are preprogrammed. They are to be used
as guidelines.
Each protocol has a 1 hour, 1000 V hold step to maintain sharp
bands once focusing is complete. This step is not necessary and can
be removed. It can also be extended as desired.
Program 1
Focusing of most 7 cm IPG’s by limiting volt parameters.
Name: 7 cm broad&basic
Delay 0:00, Delay temp 20 °C, Run temp 20 °C, 500 μA, 6000 V, 0.5 W
Step 1, Gradient volt, 500 V, 0:30 Hrs
Step 2, Gradient volt, 1000 V, 0:30 Hrs
Step 3, Gradient volt, 6000 V, 0:30 Hrs
Step 4, Constant volt, 6000 V, 8000 Vhrs
Step 5, Constant volt, 1000 V, 1:00 Hrs
Program 2
Focusing of 7 cm IPG’S under constant watt conditions.
Name: 7 cm const watt
Delay 0:00, Delay temp 20° C, Run temp 20 °C, 500 μA, 6000 V, 0.5 W
Step 1, Constant watt 0.1 W, 1:00 Hrs
Step 2, Constant watt 0.5 W, 8000 Vhrs
Step 3, Constant volt, 1000 V, 1:00 Hrs
Program 3
Focusing of broad and medium 18 cm IPG’s by limiting volt parameters.
Name: 18 cm broad&basic
Delay 0:00, Delay temp 20°C, Run temp 20°C, 500μA, 12000V, 1.5W
Step 1, Gradient volt, 1000V, 1:00 Hrs
Step 2, Gradient volt, 12000V, 1:00 Hrs
Step 3, Constant volt, 12000V, 25000Vhrs
Step 4, Constant volt, 1000V, 1:00 Hrs
Program 4
Focusing of 18 cm narrow pH range IPG’s by limiting volt parameters.
Name: 18cm narrow
Delay 0:00, Delay temp 20 °C, Run temp 20 °C, 500 μA, 12000 V, 1.5 W
Step 1, Gradient volt, 1000 V, 1:00 Hrs
Step 2, Gradient volt, 12000 V, 1:00 Hrs
Step 3, Constant volt, 12000 V, 50000 Vhrs
Step 4, Constant volt, 1000 V, 1:00 Hrs