Hoefer IEF100 - Manual (Page 53)

• p47
Program 5
Focusing of 18 cm IPG’S under constant watt conditions.
Name: 18 cm const watt
Delay 0:00, Delay temp 20 °C, Run temp 20 °C, 500 μA, 12000 V, 1.5 W
Step 1, Constant watt 0.1 W, 1:00 Hhrs
Step 2, Constant watt 1.5 W, 25000 Vhrs
Step 3, Constant volt, 1000 V, 1:00 Hrs
Program 6
Focusing of broad and medium range 24 cm IPG’s by limiting volt
parameters.
Name: 24 cm broad&basic
Delay 0:00, Delay temp 20 °C, Run temp 20 °C, 500 μA,
12000 V, 2.0 W
Step 1, Gradient volt, 1000 V, 1:00 Hrs
Step 2, Gradient volt, 12000 V, 1:00 Hrs
Step 3, Constant volt, 12000 V, 45000 Vhrs
Step 4, Constant volt, 1000 V, 1:00 Hrs
Program 7
Focusing of 24 cm narrow pH range IPG’s by limiting volt parameters.
Name: 24 cm narrow
Delay 0:00, Delay temp 20 °C, Run temp 20 °C, 500 μA, 12000 V, 2.0 W
Step 1, Gradient volt, 1000 V, 1:00 Hrs
Step 2, Gradient volt, 12000 V, 1:00 Hrs
Step 3, Constant volt, 12000 V, 100000 Vhrs
Step 4, Constant volt, 1000 V, 1:00 Hrs
Program 8
Focusing of 24 cm IPG’S under constant watt conditions.
Name: 24 cm const watt
Delay 0:00, Delay temp 20 °C, Run temp 20 °C, 500 μA, 12000 V, 2.0 W
Step 1, Constant watt, 0.1 W, 1:00 Hrs
Step 2, Constant watt, 2.0 W, 45000 Vhrs
Step 3, Constant volt, 1000 V, 1:00 Hrs
Program 9
Slow, focusing of 24 cm IPG’S with sample loaded into a cup. Gently
increases volt over time. Sample entry phase is prolonged and designed to
be an overnight run.
Name: 24 cm cup load
Delay 0:00, Delay temp 20 °C, Run temp 20 °C, 500 μA, 12000 V, 2.0 W
Step 1, Gradient volt, 1000 V, 4:00 Hrs
Step 2, Gradient volt, 12000 V, 6:00 Hrs
Step 3, Constant watt, 2.0 W, 64000 Vhrs
Step 4, Constant volt, 1000 V, 10:00 Hrs