Hoefer IEF100 User Manual
Page 62
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• p56
Agarose Overlay
1% Agarose in 1X Electrophoresis Buffer
Prepares 100 ml
1% agarose, 25 mM Tris, 192 mM glycine, 0.1% SDS
Prepare in a 500 ml flask to allow room for foaming.
Final Concentration
Amount
Agarose
1%
1 g
10X Electrophoresis Buffer
1X
10 ml
(250 mM Tris, 1.92M Glycine, 1% SDS)
Bromophenol blue
3 mg
Deionized water
to 100 ml
Gently swirl to suspend agarose.
Heat at low power in a microwave oven until agarose is fully dissolved.
Store in 1.5 ml aliquots at 4 °C in plastic screw top tubes.
Reheat aliquots in heating block.
Caution! SDS may cause the solution
to boil over so exercise caution when
heating and prevent boiling over.
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