Hoefer PR625 Immunoblot - Manual (Page 11)

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p9
problem possible causes recommendations
Bubbles
( Note: Small bubbles
generally move when
the unit is rocked
and do not usually
affect results.)
Solutions were too cold.
Dissolved gas emerges from the
solution as the temperature rises.
Bring solutions to room temperature
before loading samples.
Channels contained residual
drops of liquid before samples
were loaded. Tilt the unit and aspirate liquid from
the lower end of each channel with
a plastic pipette tip attached to a
strong vacuum.
Improper pipetting technique
was used. Seat the pipette tip firmly in the
numbered hole and dispense the
sample with a single smooth action.
Sealing pads were moist before
mounting. Ensure that sealing pads are dry before
mounting them on the ImmunoBlot.
Poor quality or poorly maintained
pipette introduced bubbles into
the channels. Try injecting samples with a different
pipette or brand of tip.
Bubbles formed over time. Place a layer of plastic wrap between
membrane and sealing pad.
Manifold is difficult
to insert. O-rings may need lubrication
or replacement. To lubricate:
• Remove O-rings carefully with the
tip of a flat weighing spatula.
• Apply silicone grease lightly.
• Reinstall.