Multichannel Systems USB-MEA32-STIM4 Manual - Manual (Page 26)

USB-MEA32-STIM4 Manual
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4.4.2 Perfusion
A PH01 perfusion cannula is already integrated on top of the USB-MEA32-STIM4-System.
Connected to a TC01/02 temperature controller, it is possible to keep the temperature of the
perfusion medium constant at the desired value. There is no heating from below as with the
regular MEA amplifiers.
To connect the perfusion cannula PH01, please read PH01 manual for detailed information.
Bath or test solutions can be applied at flow rates from 500 μl to 4.5 ml per minute. The PH01
can be connected to any tubing that fits to the perfusion inlet (luer lock) and outlet (1 mm OD
stainless steel cannula). A titanium wire inserted into the cannula results in a turbulent flow that
ensures that the temperature of the liquid is constant across the flow stream. For setting up a
complete perfusion system, a pump and a perfusion port for aspirating the liquid from the culture
chamber are required. Standard setups for MEA recording use a peristaltic pump or syringe pump,
and a standard metal cannula with beveled tip for aspiration. The height of the aspiration needle
can be adjusted with a setscrew. The setscrew is the screw closest to the tip of the needle. Make
sure to ground both the PH01 and the aspiration needle.
The PH01 is equipped with a standard platinum temperature sensor (PT 100). The fluid
temperature is controlled by the connected temperature controller TC01/02. Please read
PH01 and TCX manual for more information.