Nova-Tech Orbeco-Hellige Legionella Single Test Kit - Manual (Page 4)

Resuspend the Bacteria
Disconnect the filter from the syringe. Discard the large syringe. The hollow fiber filter has a white cap on the
opposite end of the side used for attaching to the syringe. Remove this cap and swap ends, so that the cap is
now screwed into the place where you attached the sampling syringe. By twisting the lock ring and pulling the
filter from the fitting.
Now, take a small syringe of recovery buffer, remove the red cap and attach it to the open end of the hollow
fiber filter.
• Pull the syringe back to the 0.5 ml mark to suspend the recovery buffer, then push the syringe all
the way to the 0 ml mark.
• Repeat this process two (2) additional times.
• Draw the syringe back to collect 0.1 ml of sample, then disconnect from the filter. The syringe
now contains the recovered bacteria ready for testing.
Add Sample to the Test Strip
Remove the test from its foil wrapping, and place the test on a flat surface.
The foil wrapping should not be opened until immediately prior to running the test. If the foil is opened and
then the test is not performed within 60 minutes, discard the test.
Before use, the test should have 2 pale blue lines across the result window. If these are not present, notify your
supplier to replace the test kit. Take the pipette from the foil wrapping.
Place the syringe over the small sample window at the end of the test strip, and press the plunger so that 0.1 ml
of sample is dispensed onto the test strip. Record the time.
Take the Sample
Before taking the sample, flush the sample port for 15 seconds. Collect a water sample of at least 250 ml in a
clean container.
From the kit, take a 60 ml syringe and draw up 50-60 ml of the sample. Remove the Hollow Fiber Filter from the
packaging and attach it to the luerlock edge of the syringe. Filter the sample over a sink or other waste water
outlet. Repeat this process until all of the original 250 ml sample has been filtered. This should take no longer
than 10 minutes.
Test Procedure - Filtered Test for Water Samples
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