Read the test strip, Interpreting the results – Nova-Tech Orbeco-Hellige Legionella Single Test Kit User Manual

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Example of a new test

Example of a positive test

Example of a negative test

Read the Test Strip

Leave the test strip sitting on a flat surface during incubation. For optimum results the test results should be
performed at room temperature. After waiting 25 minutes, examine the test strip in good lighting. If the
test is not read within 60 minutes of adding the water sample, it should be discarded and a new test should be
run. The test should show one of the following results on the large result window on the test strip:

Negative Result: One (1) RED line across the result window at the end furthest from the sample window.

Positive Result:
Two (2) RED lines across the result window. The red line closest to the sample window may
be very faint (pale pink). Any distinct line, no matter how faint, should be counted as positive.

Invalid Result:

If the test does not show any red lines; or if it only shows a line at the end closest to the sample

window; or if the line furthest from the sample window is very faint, then the test is invalid. Repeat the test and
notify your supplier for technical support.

Interpreting the Results

Positive Results:

A positive test result indicates that Legionella pneumophila serogroup 1 bacteria were present in the sample
above the detection limit.

The test does not differentiate between viable (living) and non-viable (dead) organisms. The test will detect
viable but non-cultruable bacteria which are not detectable by traditional laboratory techniques. A positive
result does not necessarily mean that viable bacteria are present.

When a positive result is observed, seek advice from your risk management plan, or water treatment specialist.

Negative Results:

A negative test result indicates that Legionella pneumophila serogroup 1 bacteria were not detected or the
number of bacteria in the sample were below the detection limit.

A negative result does not necessarily mean that Legionella bacteria are absent.

A negative result does not mean that the system is completely free from risks associated with Legionella
bacteria. The test only detects Legionella pneumophila serogroup 1. The test does not detect the presence of
other Legionella species or serogroups.

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