Eppendorf Piezo-actuated Mouse ICSI (intracytoplasmic sperm injection) - Manual (Page 3)

Sperm
droplet Sperm head
droplet
Userguide No 037 | Page 3
4. 1 Installation of PiezoXpert onto TransferMan NK 2
Loosen the M3x12 cheese head screw and remove the X
head (Figure 5A). Rotate the X head 180°.
Tighten the X head again using the M3x12 cheese head
screw.
Loosen the knurled screw (Figure 5B). Remove the knurled
screw and the pressure plate from the X head.
Place the provided spacer plate on the bore of the X head.
Secure the spacer plate using the knurled screw and pres-
sure plate.
Fig. 4: ICSI dishes for sperm head isolation (A) and injection (B) using piezo-assisted method
Fig. 5: Installation of spacer plate onto TransferMan NK 2
Place the actuator in the upper (see Figure 6A) or lower (see
Figure 6B) groove of the distance plate. Tighten the knurled
screw to secure the actuator between the distance plate
and pressure plate.
The flatter the angle of the capillary, the more direct the ef-
fect of the piezo impulses. Most people use straight or low
angled capillaries for piezo-actuated injection.
Injection droplets
Sperm head droplet
Injection droplets
A
B
A
B
Fig. 6: Installation of piezo actuator onto TransferMan NK 2
Connect the tubing from CellTram vario to the rear end of
the capillary holder of the actuator.
Dispense the oil by rotating the CellTram vario knob to the
right until it is dripping from the opening of the grip head.
A B
4. Equipment setup