Leica Biosystems RM CoolClamp - Manual (Page 14)

Instrument Setup
INHECO GmbH 14
Prerequisite for sectioning the specimens
Prerequisite for using the RM CoolClamp:
To use it with the rotary microtomes of the RM22/23.. series, a vertical travel
range of at least 70mm and an adjustable force balance are necessary.
To use the non-directional specimen fixture or the directional specimen fixture
(fine or only XY), bring the specimen fixture into the zero position.
The embedded tissue samples to be sectioned must be precooled to -5 °C
[+23 °F] using ice or a cooling plate prior to sectioning.
After the RM CoolClamp's precooling phase of 30 min., the universal cassette
can be inserted and the sections carried out.
Permitted maximum sectioning speed 420 mm/s
Section thickness range 0.5
– 600 µm
Operating concept
Installation on a rotary microtome of the RM22/23.. series:
The following applies in general: Handling specimen head or knife / blade
holder, always lock handwheel in place. Release locking mechanism only
for sectioning and use knife guard; see Safety Notes on page 8.
For a microtome that is already configured
– remove knife / blade holder with
base and specimen clamp. Bring the specimen head into the rear end position
(home position). Lock the handwheel in place and, where appropriate, check
whether the handbrake has been activated.
We recommend working with the non-directional specimen holder fixture
– if
you will be working with the directional specimen fixture (XY or fine), it must be
brought into the zero position.
Push the RM CoolClamp with adapter into the dovetail guide of the quick
change system for specimen clamps and tighten it using a size 4 Allen key.
To carry out the force balancing, please observe the operating manual of the
respective microtome under the Operation chapter, Fine Adjustment of the
Force Balance. The specimen head must not fall into the knife after being
stopped/released.
Reinstall the knife / blade holder with base and select the desired clearance
angle.
Attach the cable holder to the microtome; cable holders are included in the
standard delivery of the RM CoolClamp.
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