SciGene Hybex Microarray Incubation System - Manual (Page 9)

Hybex® Microarray Incubation System — USER MANUAL
Version 1.1E, August 2012 8 (408) 733-7337, [email protected]
E. Calibrating the EZ-Zone Temperature Controller
Note: For instruments manufactured prior to July 2012, instructions for calibrating the SD-31
controller can be found in the Appendix of this document.
The temperature controller comes calibrated from the factory to provide accurate block
temperatures from 35 to 99°C.
The temperature controller will require calibration only if:
• When checking the block temperature with a calibrated digital thermometer, the
thermometer temperature differs by more than one degree (1°C) from the
temperature shown on the controller display.
A NIST calibrated digital thermometer (sold separately, SciGene Cat. #1051-52-0) is required
to calibrate the unit.
Follow these steps to adjust the controller to achieve accurate temperatures.
1. Insert a block into the Hybex® incubator, turn ON the
instrument and set the controller to 65°C. Allow 15
minutes for temperature to stabilize.
2. Using the cable provided with the digital thermometer,
plug one end into the blue receptacle found on the
back panel of the Hybex® incubator and the other end
into the digital thermometer.
3. Turn on the thermometer. Allow 1 minute for
thermometer to stabilize. The actual temperature of
the block will be displayed.
4. Calculate the difference between the temperature
shown on the EZ-Zone controller and on the digital
thermometer. For example, if the controller displays
65.0°C and the thermometer displays 66.5°C, the
difference (offset) is 1.5°C.
5. On the controller, press the up and down arrows simultaneously for 3 seconds. The
left display shows “A1” and the right display shows “oPEr”.
6. Press the Advance Button (green circle) 3 times until the right display shows “i.CA”.
The left display will show the offset value between the controller and thermometer
when the unit was last calibrated.
7. Use the up or down arrow to adjust the offset value to the temperature difference
calculated in Step 4. For example, if the controller shows a temperature 1.5°C lower
than the thermometer, adjust the offset by adding 1.5 to the value shown.
8. Press the Infinity Key (∞) twice to exit calibration and return to the operation
display. Verify that the temperature on the thermometer matches the display. Your
incubator is now calibrated to provide accurate temperatures between 35 and 99°C.