UVP Doc-It Life Science - Manual (Page 84)

Performing 1D Analysis
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and quite distinct. Joined Valleys requires a sensitivity value to be entered. A higher value of sensitivity
starts "eating" into the bands, which may not be accurate.
To Use Joined Valleys:
Select Analysis > 1D Analysis > Background Correction > Joined valleys or use the 1D
Analysis Plugin to select Joined valleys in the dropdown menu.
A pop-up window appears that allows the user to set a sensitivity value from 0 to 100. Change
the size either by typing in the sensitivity value, or by using the up and down arrow signs. Click
OK after entering the radius
Rolling Disc
Picture turning the lane profile graph upside and then rolling a ball over the new top. Everything the ball is
able to roll over is eliminated by the software; whatever the ball cannot roll over remains in the graph for
analysis. Rolling disc performs well in all background conditions providing the size of the disc is carefully
chosen. An excessively small disc will "roll into" bands, eliminating the band data almost entirely. An
excessively large disc rolls across the lowest valleys, acting much like Straight Line correction.
To Use Rolling disk:
Select Analysis > 1D Analysis > Background Correction > Rolling disk or use the 1D
Analysis Plug-in to select Rolling disk from the dropdown menu.
A pop-up window appears that allows the user to set a Rolling Disk Radius value from 1 to
1000. Change the size either by typing in the sensitivity value, or by using the up and down
arrow signs. Click OK after entering the radius
Area Between Lanes
Part of the image may be overexposed, and there may be patterns of deformity between the lanes. This
correction takes cross-sections between lanes and subtracts those "inter-lane" profiles. Area Between
Lanes performs well in all variable illumination situations, providing lanes are distinct and there are clear
gaps between them. It performs badly if bands in different lanes "bleed together" or touch, because it will
tend to eliminate almost all band data at such a point.
Tip: For better concentration calibration accuracy, it is recommended the band
boundaries/ extents be reviewed and adjusted if necessary. Related topic: Band
adjustment. See Finding Lanes and Bands.
Concentration Calibration
Showing the Concentration Graph
With the background of the image corrected, LS software is now ready to graph intensity versus
concentration and to fit curves or lines on the graph. It also allows the user to change the Unit Type
plotted on the y-axis.
To Show the Concentration Graph:
From the Analysis > 1D Analysis > Master Tools > Concentration or click on Concentration in
the 1D Analysis Plug-in. A new window appears with a blank graph.
Graph Options