UVP Doc-It Life Science - Manual (Page 89)

Performing 1D Analysis
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In the Step 1: Select MW standard tab select the standard to copy. Then click on Copy.
The Edit window will appear. Change the Name of the new MW Standard. Click on Add to enter
a new Value or click on a value and click Edit to make changes in the copy. Then click on OK.
Applying a Molecular Weight Standard to a Lane
Calibrating a Lane
Select the lane to calibrate.
From the Analysis > 1D Analysis > Master Tools > Molecular Weight or select Molecular
Weight from the 1D Analysis Plugin.
A new Molecular Weight window opens. On tab Step 1: Select MW standard choose the
standard to use. If the standard is not on the list, add the standard to the list.
Click Next. A new screen appears Step 2: Calibrate MW standard.
In the Step 2 screen, the weights can be adjusted to match the bands that actually appear.
Complete instructions for this appear below.
Click OK to save the calibration.
Using the Stretch Factor
The stretch factor establishes a mathematical relationship between the weights to describe their relative
movement. The larger the stretch factor, the lighter weights move in relationship to heavy ones. The
smaller the stretch factor, the less the lighter weights move. A stretch factor of 1.0 indicates linear
movement (weights move in direct proportion to their relative weights).
To adjust the weights to match the bands, choose to use the stretch factor:
In the Step 2 screen of the Molecular Weight tool, ensure that Proportional calibration mode is
selected.
Drag a known weight up or down with the mouse until it matches the appropriate band.
Alternatively, select the weight with the mouse (or TAB key) and move the weight up and down
with the UP and DOWN arrows on the keyboard (this helps adjust the weight by small amounts).
When there is a match of the weight with the band, the line color changes from blue to yellow
(default colors).
Adjust the stretch factor (scaling) between weights until the other weights match their appropriate
bands. Adjust it using the mouse wheel (rolling up increases the value, rolling down decreases it),
or enter a new value into the text box and click Set.
Click OK to save the calibration.
Note: Using the Stretch Factor gives a weight match on the band up to 0.5% of the
assigned weight. To obtain exact placement on the band, use the manual mode,
described in the Using Manual Placement of Weights below.