UVP Doc-It Life Science - Manual (Page 93)

Performing 1D Analysis
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Overview
Dendrograms are graphical/numerical displays used to show the similarity bands. The similarity is
grouped into clusters. Each lane in an image has its own cluster. Then, repeatedly, a linkage rule
(selected by the user in the software) is employed to merge smaller groups into larger clusters, until all
the clusters have been combined into a single cluster. The result is a hierarchy of clusters. When moving
up the hierarchy, each cluster contains more but less similar lanes. Lanes that are very similar to each
other will appear together in clusters near the bottom of hierarchy.
This section will explain how to:
Automatically generate dendrogram information
Automatically generate multi-image dendrogram information
Modify dendrogram graphs
Generating Dendrogram Graphs
After finding the lanes and bands in the image the user may begin the process of generating dendrogram
graphs.
Finding lanes and bands must be performed first.
Go to Analysis > 1D Analysis > Dendrograms or select Dendrograms from the 1D Analysis
Plugin.
A Dendrogram View window will appear.
The user may select from Band Formula: Rf Values or Weight Values, Lane Formula: Dice
Similarity or Jaccard Similarity and the Linkage Formula.
A Dendrogram View Graph will appear detailing the relationships between lanes and bands.
Generate Multi-Image Dendrogram Graphs
Finding lanes and bands must be performed first on all images to be used in the graph. Related
topic: Band adjustment.
Go to Analysis > 1D Analysis > Multi-Image Dendrograms or select Multi-Image Dendrograms
from the 1D Analysis Plugin.
A Dendrogram MultiImage will window will appear. Click Add to select the images on which to
perform the multi-image dendrogram calculation.
Click OK.
A Dendrogramv View window will appear.
The user may select from Band Formula: Rf Values or Weight Values, Lane Formula: Dice
Similarity or Jaccard Similarity and the Linkage Formula.
Modify Dendrogram Graphs
The dendrogram graph allows users to change the Background color, Line Color, Node Color, and Text
Font. The user may also select to change the lane identification type to Names or Names and IDs.
Simply use the dropdown menu in each category to select the desired graphical display option.