LaMotte AM-21 Water Pollution Detection Outfit - Manual (Page 45)

Overhead Projection Demonstration
Quantity Contents Code
15 mL *Sulfide Reagent A *4458-E
15 mL Sulfide Reagent B 4459-E
30 mL Sulfide Reagent C 4460-G
1 Test Tube, 5.0 mL 0230
1 Pipet, 1.0 mL 0354
1 Demonstration Stage, six cell 1038
1 Spatula 0691
WARNING: Reagents marked with a * are considered to be potential health hazards.
To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD
or www.lamotte.com. To obtain a printed copy, contact LaMotte by email, phone or fax.
Procedure
1. Place the demonstration stage (1038) on the overhead projector and
turn on the projector light.
2. Fill test tube (0230) to the 5 mL line with sample water and transfer this
liquid to a cell on the demonstration stage (1038).
3. Add 15 drops of *Sulfide Reagent A (4458) to the sample in the cell.
Stir with the spatula (0691). Remember that this is a strongly acidic
solution.
4. Add 3 drops of Sulfide Reagent B (4459) and stir. Wait one minute.
5. Add 1.0 mL of Sulfide Reagent C (4460) and stir the contents of the
cell.
6. If sulfide is present, a blue color will develop. Traces of sulfide will
produce faint blue colors and high concentrations will produce deep blue
colors.
45