LaMotte Aquaculture TEST KIT AQ-1 - Manual (Page 4)

NITRITE NI TRO GEN TEST
NOTE: Place dispenser cap (0692) on *Mixed Acid Reagent (V-6278).
Save this cap for refill reagents.
1.
Fill a square tube (0106) to the 2.5 mL line with sample water.
2. Dilute to 5 mL line with *Mixed Acid Reagent (V-6278).
3. Use the 0.1g spoon (0699) to add 0.1g of *Color Developing Reagent
(V-6281). Cap and mix for one minute.
4. Face natural light. Holding the Nitrite
Nitrogen ColoRuler (3351) in an upright
position, place the bottom of the tube on
the ridge above the standards. Match
sample color to a color standard. Record as
ppm Nitrite Nitrogen.
NOTE:
If the resulting color is darker than
the highest standard, a dilution is required.
Follow the dilution procedure below.
DILUTION PROCEDURE
1. Use the 0.5 mL pipet (0353) to add 0.5 mL of sample water to a
second square tube (0106).
2. Dilute to 5 mL line with deionized water.
3. Use 2.5 mL of the diluted sample and follow the instructions above,
starting with Step 2. Multiply result by 10. Record as ppm nitrate
nitrogen.
4. If the color is still darker than the highest standard use the 0.5 mL
pipet (0353) to add 0.5 mL of the sample to a tube and dilute to 10
mL.
5. Use 2.5 mL of this diluted sample and follow instructions starting with
Step 2.
6. Multiply result by 20. Record as ppm Nitrite Nitrogen.
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pH
1. Fill a square tube (0106) to the 5 mL line with sample water.
2. Add 8 drops of *Wide Range Indicator (2218). Cap and mix.
3. Hold the tube against the white area of the pH Color Chart
(3603-CC). Match sample color to a color standard. Record the results
as pH.
DIS SOLVED OX Y GEN TEST
COLLECTING THE WATER SAMPLE
1. To avoid contamination, rinse the Water Sampling Bottle (0688-DO)
thoroughly with sample water.
2. Tightly cap the bottle. Submerge to the desired depth, and remove the
cap to allow the bottle to fill.
3. Tap the sides of the submerged bottle to dislodge any air bubbles
clinging to the inside of the bottle. Replace the cap while the bottle is
still submerged.
4. Retrieve the bottle. Examine it carefully to make sure that no air
bubbles are trapped inside. Once a satisfactory sample has been
collected, proceed immediately with Steps 5 and 6 to “fix” the sample.
“FIXING” THE WATER SAMPLE
NOTE: Be careful not to introduce air into the sample
while adding the reagents in Steps 5 and 6. Simply drop
the reagents into the test sample, cap carefully, and mix
gently.
5.
Add 8 drops of *Manganous Sulfate Solution
(4167) and 8 drops of *Alkaline Potassium Iodide
Azide Solution (7166). Cap and mix by inverting
gently several times.
A precipitate will form. Allow the precipitate to
settle below the shoulder of the bottle before
proceeding.
6. Use the 1.0g spoon (0697) to add one level
measure of *Sulfamic Acid Powder (6286). Cap and gently shake to
mix, until both the reagent and the precipitate have dissolved. A clear
yellow to brown-orange color will develop, depending on the oxygen
content of the sample.
NOTE:
Following the completion of Step 6, contact between the
water sample and the atmosphere will not affect the test result. Once
the sample has been “fixed” in this manner, it is not necessary to
perform the actual test procedure immediately.
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