LaMotte NITRATE NITROGEN KIT - Manual (Page 2)

PROCEDURE
LOW RANGE (0-1.0 ppm Nitrate Nitrogen)
1. Fill the water sampling bottle (0688) with sample water.
2. Fill one test tube (0898) to the lower line (5 mL) with sample water.
3. Dilute to second line with *Mixed Acid Reagent (V-6278). Cap and mix.
4. Wait 2 minutes.
5. Use the 0.1 g spoon (0699) to add one level measure (avoid any excess) of
*Nitrate Reducing Reagent (V-6279).
6. The mixing procedure is extremely important. Cap tube. Invert tube slowly and
completely 30 times in 1 minute to insure complete mixing.
7. Wait 10 minutes.
8. Insert test tube into Low Range Comparator (1102) with Nitrate-Nitrogen Low
Range Comparator Bar (3614-01). Fill one test tube (0898) to the 10 mL line with
sample water. Place in Low Range Comparator (1102). Match sample color to a
color standard. Record as ppm Nitrate-Nitrogen.
NOTE: To convert to nitrate, multiply by 4.4. Record as ppm Nitrate.
HIGH RANGE (0-10.0 ppm Nitrate Nitrogen)
1. Use the 0.5 mL pipet (0353) to add 0.5 mL of the water sample to a test tube
(0898).
2. Add Distilled Water to the lower line (5 mL).
3. Dilute to second line with *Mixed Acid Reagent (V-6278). Cap and mix.
4. Wait 2 minutes.Use the 0.1 g spoon (0699) to add one level measure (avoid any
excess) of *Nitrate Reducing Reagent (V-6279).
5. The mixing procedure is extremely important. Cap tube. Invert tube slowly and
completely 30 times in 1 minute to insure complete mixing.
6. Wait 10 minutes.
7. Insert test tube into Low Range Comparator (1102) with Nitrate Nitrogen Low
Range Comparator Bar (3614-01). Fill one test tube (0898) to the 10 mL line with
sample water. Place in Low Range Comparator. Match sample color to a color
standard. Multiply the reading by 10. Record as ppm Nitrate-Nitrogen.
NOTE: To convert to nitrate, multiply by 4.4. Record as ppm Nitrate.