Bio-Rad Precision Melt Analysis™ Software - Manual (Page 45)

Analyzing Melt Data
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Changing Cluster Parameters
Right-click on the fluorescence trace of a well in the Melt Curve or Difference Curve chart to
change cluster parameters (Figure 30).
Figure 30. Changing cluster parameters.
Changes made to the cluster are applied to all samples in the cluster. Select from the options
listed in the Selected Cluster menu:
• Select <Set Cluster Name> to assign a name to the cluster. Enter the name of the
cluster in the Rename window text box and click OK
• Select <Set Cluster Color> to change the color of the fluorescence traces for the
wells assigned to the selected cluster. Choose the new color from the color options
in the Color window and click OK
• Select <Set as the Reference Cluster> to use the selected cluster as the Difference
Curve reference cluster
Precision Melt Tab Spreadsheet
Table 15 lists the data shown in the spreadsheet at the bottom right of the Precision Melt tab.
TIP: To make changes to the Content and Sample, open the Plate Editor by clicking
View/Edit Plate.
Table 15. Precision Melt tab spreadsheet contents
Information Description
Well Well position in the plate
Content A combination of the Sample Type and Replicate # loaded in
the well in the Plate Editor
Sample Sample name loaded in the well in the Plate Editor
Cluster Name of the cluster assignment for the well
Confidence Indication of the relative probability the sample has of being in
a cluster