Bio-Rad Helios® Gene Gun System - Manual (Page 19)

Assembly of the Tubing Prep Station and Syringes
Components needed
Tubing Prep Station, base
Tubing Prep Station, tubing support cylinder
Tubing Prep Station, power cord
O-rings, for tubing support cylinder
Syringe adapter tubing (silicone, 5 ft, 0.104" ID, 0.192" OD)
10 cc syringes (2)
10 cc syringe sleeve
1/8" barb-to-male Luer fittings (2)
1/8" barb-to-female Luer fittings (2)
Scissors, user supplied
Fig. 11. Components and controls on the Tubing Prep Station, fully assembled.
Procedure
1. Place an O-ring in the stainless steel end of the tubing support cylinder next to the spur gear.
2. Insert the end of the tubing support cylinder with the spring into the hole in the support bar
on the base of the Tubing Prep Station. Push the tubing support cylinder to compress the
spring, then slide the end of the tubing support cylinder containing the O-ring into the sup-
port on the motor housing. (Note: Carry the Tubing Station by the base, not by the tubing
support cylinder.)
3. Cut a 4–5" piece of syringe adapter tubing; attach each end to the barb of the 1/8" barb-to-
male Luer fittings. Attach one of the male Luer-Lok fittings to the female Luer on the top of
the flowmeter. Attach the other male Luer-Lok fitting to the female Luer on the side of the
motor housing on the Tubing Prep Station.
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