Bio-Rad Helios® Gene Gun System - Manual (Page 41)

Possible solutions 1. Make sure DNA is resuspended in TE or distilled water, not saline.
2. Check that DNA has been precipitated onto the microcarriers
(see Section 10.7).
Problem Attached cells are lifting off the center of the plate after delivery
of gold particles, or there is a dead zone in the center of the target.
Possible solutions 1. Lower the helium pressure and/or lower the microcarrier
loading rate.
Problem Contamination of the cell culture.
Possible solution Wipe the end of the barrel with ethanol or dilute bleach.
Section 9
Product Information
9.1 Helios Gene Gun System
The two catalog numbers for the two Helios Gene Gun Systems (100/120V and 220/240V)
and component descriptions are as follows:
Catalog
Number
Product Description
165-2431 Helios Gene Gun System, 100/120 V includes
165-2411 Helios Gene Gun Kit, that includes
Gene Gun Unit, 1
165-2416 O-rings, Helios Gene Gun, 5
165-2417 Barrel Liner, 5
165-2426 Cartridge Holder, white, 5
165-2435 Cartridge Extractor Tool, 1
165-2436 Battery, 9 V, 1
165-2412 Helium Hose Assembly, with Swagelock Quick-Connect fittings, 1
165-2413 Helium Regulator, CGA 580 female fitting, with pressure relief
valve. Maximum pressure 2,600 psi., 1
165-2418 Tubing Prep Station, includes Tubing Prep Station, 100/120 V,
Tubing Support Cylinder, 1 Power Cord, 1, O-rings, Tubing Prep
Unit, 5 (165-2419), Nitrogen Regulator Hose, Nalgene, 12 ft,
3/16” Barb to Male Luer-Lock Fitting, 2, Nitrogen Flow Meter Fitting,
1/8” Barb-to-Male Luer-Lock Fitting, 2, Allen wrench, 5/64”, 1,
Syringe Holder, 10 cc, 1
165-2421 Syringe Kit , 1, includes: Syringe Adaptor Tubing, Silicone, 5 ft,
0.104” ID x 0.192” OD, 1, Syringe 10 cc, 5, Syringe Adaptor
Fitting, 1/8” Barb-to-Female Luer-Lock Fitting, 5
165-2422 Tubing Cutter , Helios Gene Gun, that includes Tubing Cutter Unit
and Razor blades, 10 (165-2423)
165-2424 Helios Gene Gun Optimization Kit, that includes
165-2262 0.6 micron gold, 0.25 g
165-2263 1.0 micron gold, 0.25 g
165-2264 1.6 micron gold, 0.25 g
165-2440 Cartridge Kit, Helios Gene Gun, 1, that contains PVP
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