Bio-Rad Mini-PROTEAN® 3 Dodeca™ Cell - Manual (Page 10)

Problem Cause Solution
b. Ionic strength of sample b. Use same buffer in
lower than that of gel sample as in gel or
stacking gel.
8. Skewed or distorted bands a. Poor polymerization a. Degas stacking gel
around the sample wells solution thoroughly
prior to casting;
increase ammonium
persulfate and TEMED
concentration by 25%,
wipe comb with
TEMED just before
casting the stacking gel,
insert comb carefully to
prevent trapping of air
bubbles in sample well.
b. Salts in sample. b. Remove salts by
dialysis, desalting
column, etc.
c. Uneven gel interface c. Decrease the
polymerization rate.
Overlay gels very
carefully.
9. Lanes constricted at the a. Ionic strength of sample a. Desalt sample and
bottom of the gel. higher than that of neighboring samples.
surrounding gel.
Section 5
Product Information and Accessories
Catalog
Number
Description
Mini-PROTEAN 3 Dodeca Cell and Accessories
165-4100 Mini-PROTEAN 3 Dodeca Cell, includes 6 clamping frames,
2 buffer dams, drain line, and 2 gel releasers
165-4101 Mini-PROTEAN 3 Dodeca Cell, with multi-casting chamber, (includes
165-4100 and 165-4110, order glass plates and combs separately)
165-4102 Replacement clamping frame, 1
165-4103 Lower electrode assembly with platinum wire
165-4104 Replacement drain line
165-4105 Replacement cooling coil, includes connector tubing
165-2948 Replacement power cables
165-3130 Buffer dam
8