Bio-Rad Mini-PROTEAN® 3 Dodeca™ Cell - Manual (Page 5)

Section 2
Setting up the Dodeca Cell for Electrophoresis
2.1 Buffer preparation
Prepare 3.4–4.4 liters of running buffer (minimum/maximum) for electrophoresis. Buffer
should be equilibrated to room temperature prior to electrophoresis. Premixed buffers are
available (see Section 5).
2.2 Gel Preparation
Ready Gel precast gels or handcast gels using Mini-PROTEAN 3 glass plates can be run
in the Mini-PROTEAN 3 Dodeca cell. Note: All gels in a single electrophoresis experiment
must be of the same gel type, with the same buffer and the same % acrylamide.
2.2.1 Ready Gel Precast Gel Cassette Preparation
Note: The Mini-PROTEAN 3 Dodeca cell is guaranteed only for use with Ready Gel
precast gels.
For complete Ready Gel precast gel instructions, order catalog number 161-0993, Ready Gel
Applications Guide. The Applications Guide is also available on the internet at www.bio-rad.com
in the literature section.
a. Take the Ready Gel precast gel out of its storage pouch.
b. With a razor blade, cut the tape along the black line at the bottom of the gel.
c. Pull the clear tape at the bottom of the Ready Gel Cassette to expose the bottom edge of
the gel.
Repeat for additional Ready Gel precast gels up to a maximum of 12 gels.
Note: If an odd number of gels is to be run, use the buffer dam on the opposite side of the
clamping frame with only one gel to create the clamp assembly.
2.2.2 Handcast Gel Preparation Using Mini-PROTEAN 3 Glass Plates
a. Cast gels per the instructions with the Mini-PROTEAN 3 multi-casting chamber.
b. Remove the gels from the casting chamber or from the storage container.
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