Bio-Rad PROTEAN® Plus Hinged Spacer Plates and Combs - Manual (Page 10)

Fig. 7.1. Linear gradient gel. a. 5-30% gradient. b. 10-20% gradient.
Fig. 7.2. Concave gradient—relation of heavy and light solutions in the mixing and reservoir
chambers. Intensity of shading in the slab gel sandwich indicates change in pore size.
8
a
b
% Acrylamide
Amount delivered (%)
25 50 75 100
30
25
20
15
10
5
% Acrylamide
25 50 75 100
Amount delivered (%)
a
b
5
10
15
20
25
30
Peristaltic
pump