Bio-Rad PROTEAN® Plus Hinged Spacer Plates and Combs - Manual (Page 2)

Table of Contents
Page
Section 1 Introduction..........................................................................................1
1.1 Specifications ....................................................................................................2
Section 2 Pouring a Linear Gradient Gel ...........................................................2
2.1 Calculating the Chamber Volumes ....................................................................2
2.2 Pouring the Gel from the Top ............................................................................2
2.3 Pouring the Gel from the Bottom.......................................................................3
Section 3 Pouring an Exponential Gradient Gel ................................................4
3.1 Concave Exponential Gradient Gel....................................................................4
3.2 Convex Exponential Gradient Gel .....................................................................5
Section 4 Pouring Two Gradient Gels.................................................................5
Section 5 Pouring Multiple Gradient Gels in a Gel Casting Chamber .............6
Section 6 Gradient Former Care and Maintenance...........................................7
Section 7 Theory of Linear and Exponential Gradient Gels .............................7
7.1 Linear Gradients................................................................................................7
7.2 Exponential Gradients .......................................................................................7
Section 8 Protocols .............................................................................................10
8.1 Preparation of Stock Solutions for Laemmli SDS-PAGE Slab Gels ................10
8.2 Calculating the Volume of the Gel...................................................................11
8.3 Gel Preparation................................................................................................12
8.4 Linear Gradient Gels .......................................................................................13
8.5 Exponential Gradient Gels...............................................................................14
Section 9 Equipment and Accessories...............................................................15
9.1 Model 385 and Model 395 Gradient Former and Accessories .........................15
9.2 Additional Required Equipment ......................................................................15
9.3 PROTEAN ® II xi and Mini-PROTEAN ® II
Vertical Electrophoresis Cells and Accessories ...............................................15
9.4 Electrophoresis Chemicals...............................................................................17