Ac b – Bio-Rad Fluorescent Protein Stains User Manual
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R
2
= 0.9943
0
500
1000
1500
2000
2500
0
1
2
3
4
5
fl
u
o
rescen
ce
R
2
= 0.9947
0
5000
10000
15000
20000
25000
0
10
20
30
40
50
60
70
ng
β-galactosidase
fl
u
o
rescen
ce
R
2
= 0.9960
0
50000
100000
150000
200000
250000
300000
0
200
400
600
800
1000
1200
ng
β-galactosidase
fl
u
o
rescen
ce
A
C
B
ng
β-galactosidase
Fig. 4. Serial 2-fold dilutions of
ββ-galactosidase, run on a Criterion™ Tris-HCl 4–20% gel.
The gel was stained with Flamingo Fluorescent Gel Stain and imaged with the Molecular Imager FX
system. Fluorescence associated with the
β-galactosidase band was determined using Quantity One
®
1-D Analysis Software. A
A: Fluorescence associated with protein band in the range 0.25–4 ng. B
B:
Fluorescence associated with protein band in the range 4–60 ng. C
C: Fluorescnece associated with protein
band in the range 60–960 ng.
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