Bio-Rad Fluorescent Protein Stains - Manual (Page 13)

Problem Possible Cause Remedy
Speckles or blotches in the Particulate material from Filter water used to prepare
gel image water, reagents, staining fix solution and stain
tray, ambient dust and solution if laboratory water
gloves system does not have a
filter.
Make sure that the staining
trays are thoroughly clean.
Limit the time that the gels
and staining solution are
exposed to open air.
Use dust-free gloves and
handle gels only by the
edges.
Particulate material may be
washed from the surface of
the gel prior to imaging by
washing with 0.1% (v/w)
Tween 20 prior to imaging
(See Section 2.6).
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