Bio-Rad Trans-Blot® Plus Cell - Manual (Page 20)

Towbin Buffer with 10 % Methanol
25 mM Tris, 192 mM glycine, 10% v/v methanol, pH 8.3
a) Using 10X Tris /glycine buffer (catalog #161-0734 for 1L bottles or
catalog # 161-0757 for 5L cube)
100 ml of 10X Tris /glycine buffer
800 ml of deionized water (dd H
2
O)
100 ml of methanol
b) Using dry reagents:
3.03 g Tris
14.4 g glycine
600 ml deionized water (dd H
2
O)
100 ml of methanol
add dd H
2
O to 1 liter
Towbin Buffer with 15 % Ethanol
25 mM Tris, 192 mM glycine, 15% v/v ethanol, pH 8.3
a) Using 10X Tris/ glycine buffer (catalog #161-0734 for 1L bottles or
catalog # 161-0757 for 5L cube)
100 ml of 10X Tris /glycine buffer
750 ml of deionized water (dd H
2
O)
150 ml of ethanol
b) Using dry reagents:
3.03 g Tris
14.4 g glycine
600 ml deionized water (dd H
2
O)
150 ml of ethanol
add dd H
2
O to 1 liter
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