Bio-Rad Trans-Blot® Plus Cell - Manual (Page 21)

CAPS Buffer
CAPS-based transfer buffers (10 mM CAPS, 10% methanol, pH 11) may be
preferable for transfers of high molecular weight proteins (e.g. >50 000 Da) and in
cases where the glycine component of Towbin buffer may interfere with downstream
protein sequencing applications.
CAPS Buffer with 20% Methanol
10 mM CAPS (3-(cyclohexylamino)-1-propane sulfonic acid),
20% methanol, pH 11
a) Using dry reagents:
2.21 g CAPS
600 ml deionized water (dd H
2
O)
Adjust to pH 11 with NaOH
add dd H
2
O to 800 ml
200 ml methanol
CAPS Buffer with 10% Methanol
10 mM CAPS (3-(cyclohexylamino)-1-propane sulfonic acid),
10% methanol, pH 11
a) Using dry reagents:
2.21 g CAPS
600 ml deionized water (dd H
2
O)
Adjust to pH 11 with NaOH
add dd H
2
O to 900 ml
100 ml methanol
CAPS Buffer with 15% Ethanol
10 mM CAPS (3-(cyclohexylamino)-1-propane sulfonic acid),
15% ethanol, pH 11
a) Using dry reagents:
2.21 g CAPS
600 ml deionized water (dd H
2
O)
Adjust to pH 11 with NaOH
add dd H
2
O to 850 ml
150 ml ethanol
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