Bio-Rad Mini-PROTEAN II Multiscreen Apparatus User Manual
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OR:
5% BLOTTO in 100 ml of TBS
Add 5% g of BLOTTO to 100 ml of TBS.
Antibody Buffer, 200 ml
1% BSA - TTBS
Add 2 g BSA to 200 ml TTBS. Stir to dissolve.
OR
1% BLOTTO in TTBS.
Add 2 g of BLOTTO to 200 ml of TTBS.
First Antibody Solution
Dilute antigen specific primary antibody to the appropriate titer in antibody buffer.
Second Antibody Solution, 100 ml
Dilute species specific Bio-Rad second antibody enzyme conjugate, 1:3,000 by adding
33 µl of conjugate to 100 ml of antibody buffer.
Consult the Immun-Blot assay kit instruction manual for dilution protocols of the col-
loidal gold conjugates.
Color Development Solution
The specific chemicals and buffers are dependent on the enzyme conjugate being used.
See the Immun-Blot assay kit instruction manual for details on how to make the appro-
priate solution.
Section 6
References
1. Jerome, J. F. and Jaehning, J. A.,
Mol. and Cell Bio., 6, 1633 (1986).
2. Johnson, D. A., et. al., Gene Anal. Tech., 1, 3 (1984).
Eppendorf is a trademark of Eppendorf Geratebau Netheler & Hinz, GmbH
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