Bio-Rad Mini-PROTEAN II Multiscreen Apparatus - Manual (Page 8)

Fig. 3. Tilt the multiscreen apparatus toward you during sample application.
2. Wash solutions can be applied in the same manner as the antibody samples, or with the
Eppendorf repeater pipet. Use 600 µl per channel. The number and stringency of wash-
es may vary and should be determined separately for each experiment. However, a min-
imum of three washes with a buffer containing a detergent such as Tween-20 is
recommended after each antibody incubation. See Section 5 for buffer formulations.
6