Bio-Rad Sequi-Blot™ PVDF Membrane - Manual (Page 11)

Section 7
For Technical Assistance
In the U.S., technical service is available by
calling 1-800-4BIORAD (1-800-424-6723).
Our Technical Service representatives are
available to answer your questions from 8 AM
to 5 PM (PST). Technical representatives can
be contacted through your local Bio-Rad office.
For more information about the use of PVDF
membrane, request bulletin 2212.
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Section 6
Protein Detection
Staining
Proteins for sequencing can be detected
with Coomassie brilliant blue (CBB) R-250 or
with Bio-Rad’s Colloidal Gold Total Protein
Stain. CBB R-250 is the stain most commonly
used for detection of proteins prior to protein
sequence analysis. It is rapid (² 15–20 minutes),
sensitive, and does not interfere or react with
the reagents used in Edman degradation chem-
istry. See page 13 for the CBB R-250 stain
preparation without acetic acid. If a protein can
not be detected by CBB staining, there will not
be enough present to sequence. See the Blotting
for Protein Sequencing section for a protocol
for CBB R-250 staining.
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