Bio-Rad Sequi-Blot™ PVDF Membrane - Manual (Page 3)

Section 1
Introduction
The hydrophobicity of PVDF (polyvinyli-
dene difluoride) membrane makes it an ideal
support for binding proteins in electrophoretic
and dot blotting applications. Proteins are tight-
ly bound, and are quantitatively retained during
exposure to acidic, basic, or organic solvents.
1,2
Resistance to acidic and organic solvents which
would dissolve nitrocellulose or nylon mem-
branes makes PVDF membrane an excellent
support for amino-terminal protein sequenc-
ing.
1,3 The unique manufacturing process used
for Bio-Rad’s Sequi-Blot PVDF membrane
insures higher protein binding capacity than
other commercially available PVDF products.
4
Higher capacity increases the likelihood of
sequencing proteins of interest, which makes
Sequi-Blot PVDF membrane the best choice for
1
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