Bio-Rad Sequi-Blot™ PVDF Membrane - Manual (Page 4)

Section 2
Membrane Wetting
Sequi-Blot PVDF membrane can be used
as a direct replacement for the membrane cur-
rently being used in your sequencing protocol.
No changes are required in the procedure, but
the special steps given below are required to
prepare the membrane for blotting. The
hydrophobicity of the PVDF membrane makes
it impossible to wet the membrane with aque-
ous solutions. Methanol or an alternative organ-
ic solvent is required to pre-wet the membrane
prior to equilibration in transfer buffer. After
equilibration, the membrane can be used in a
semi-dry, tank, or capillary blotting system
with any acidic or basic blotting buffer.
Note: Always handle membranes using gloves or
forceps to prevent contamination.
3
sequence analysis of blotted proteins and pep-
tides.
For use of PVDF membrane as an
immunoblotting support for western blot detec-
tion, Bio-Rad offers Immun-Blot
® PVDF mem-
brane which features lower signal to
background results in this application than
Sequi-Blot membrane. However, it does not
exhibit the same high protein binding capacity,
so Sequi-Blot PVDF membrane is the best one
to use for protein sequencing.
2
LIT240c.qxd 6/23/98 11:42 AM Page 2