Bio-Rad ReadyPrep™ 2-D Starter Kit - Manual (Page 6)

4
Rehydration Setup
30 min
Rehydration Setup
30 min
IPG Rehydration
12 hours
IPG Rehydration
12 hours
IPG Rehydration
12 hours
Isoelectric Focusing
5 hours
Isoelectric Focusing
5.3 hours
Isoelectric Focusing
7 hours
IEF stain Subset of
IPGs (Optional)
60 min
IEF stain Subset of
IPGs (Optional)
60 min
IPG Equilibration
for SDS-PAGE
30 min
IPG Equilibration
for SDS-PAGE
30 min
Store IPGs at
-70 °C
Mounting IPGs
30 min
Mounting IPGs
30 min
SDS-PAGE
40 min
Destain IPG Strips
60+ min
SDS-PAGE
65 min
Bio-Safe stain
Water Wash
20 min
Rehydration Setup
30 min
Staining
60 min
Staining
60 min
Mounting IPGs
30 min
SDS-PAGE
5.5 hours
Equilibration
30 min
Destain IPG Strips
60+ min
Bio-Safe stain
Water Wash
20 min
Destaining
30-120 min
Destaining
30-120 min
Staining
60 min
Bio-Safe stain
Water Wash
20 min
Destaining
30-120 min
7 cm 11 cm 17 cm
Day 1
Day 2
Day 3
Fig. 1. Flow Chart for 2-D SDS-PAGE.