Bio-Rad ReadyPrep™ 2-D Starter Kit User Manual
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2. Repeat this process for the remaining samples by pipetting the indicated volume of
sample into adjacent channels. It is best to place samples on both sides of the tray as in
Figure 2. Maintaining the same numbering system throughout the 2-D PAGE process
helps to avoid confusing the samples. Also, during the IEF step when strips are placed on
both sides of the focusing tray, the weight of the tray lid is evenly distributed over all the
samples so contact between the IPGs and the electrode remains uniform.
3. When all the protein samples have been loaded into the rehydration/equilibration tray as
pictured in Figure 2, using forceps, peel the coversheet from one of the pH 4-7
ReadyStrip IPG strips, as in Figure 3.
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Fig. 2. Sample loading of rehydration/equilibration trays. Pipet the sample along the back edge of
the tray channel except for about 1 cm at each end. Note the even distribution of the sample along the edge
of the channel. The figure shows the last of six samples being pipeted in the tray.
Fig. 3. Removing the cover sheet from the ReadyStrip IPG strip.