Bio-Rad PROTEAN® i12™ IEF System - Manual (Page 41)

PROTEAN i12 IEF System Instruction Manual | 37
Chapter 7 Troubleshooting
Problem Cause Solution
Voltage does not increase
beyond a low value High levels of ionic contaminants
in rehydration and sample
solutions Several hours may be needed for
ionic contaminants to leave IPG
strips. Keep salt concentration
below 40 mM
Voltage does not reach the
programmed value or reaches it
very slowly Programmed voltage may not
be reached due to the sample
composition No action needed
Ampholyte concentration is
too high. Up to 1% Bio-Lyte
®
ampholytes may be used,
but ampholytes increase
conductivity; therefore, voltage
will be lower with increasing
concentrations
Lower the ampholyte
concentration
Error message appears Situation-dependent Shut down the instrument using
the power switch and then
restart it. If this fails to resolve the
issue, contact Bio-Rad Technical
Support