Bio-Rad Model 225 Tube Gel Casting Stand User Manual
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Section 1
Introduction
The benefits of performing 2-D electrophoresis in a mini-format lie primarily in
the reduced time required to complete the entire run. The IEF dimension can be
completed in 2-4 hours. The equilibration step is eliminated or minimal, and the
slab run takes only 35-45 minutes. Using Bio-Rad's protein stains (Bio-Safe
Coomassie, SYPRO Ruby protein stain, Silver stain), the whole 2-D experiment
can be completed in 1 day. Furthermore, the amount of reagents and sample used
is significantly reduced.
This manual describes the procedure for preparing and running the O'Farrell
first-dimension tube gels. For tips on sample preparation, and for alternative gel
and buffer reagent preparation, refer to the FAQs available in the technical support
section of our web site (http://discover.bio-rad.com). Refer to the Mini-PROTEAN
slab cell instruction manual for details on performing the second-dimension
SDS-PAGE electrophoresis.
Section 2
Equipment and Reagents
2.1 Equipment
Catalog
Number
Product Description
165-2960
Mini-PROTEAN 2-D Cell, includes tube cell module, 16 sample
reservoirs and stoppers, 50 sample reservoir/capillary tube
connectors, 200 capillary tubes with casting tube, tube gel ejector,
Mini-PROTEAN electrophoresis module, 2 clamp assemblies,
10 sets of glass plates with 1.0 mm spacers, 2-D combs with
1 standard well (2), casting stand with gaskets, alignment card,
leveling bubble, lid with power cables, and instructions.
165-2961
Mini-PROTEAN Tube Cell, includes tube cell module, 16 sample
reservoirs and stoppers, 50 sample reservoir/capillary tube con-
nectors, 200 capillary tubes with casting tube, tube gel ejector,
lower buffer chamber, lid and instructions.
165-2965
Mini-PROTEAN Tube Cell Module, same as 165-2961 without
lower buffer chamber and lid.
Warning:
Do not use alcohol to clean the plastic parts of the Mini-PROTEAN cell. This
will cause damage to the acrylic components. Brief exposure to 10% ethanol
at room temperature is exceptable. If you have questions about a specific
chemical compatibility, please call Bio-Rad’s technical support team (1-800-
4BIORAD)
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