Bio-Rad Model 225 Tube Gel Casting Stand User Manual
Page 17
c. Vertical streaking that is connected to a
protein spot may be caused by protein
aggregation, incomplete reduction, or
incomplete alkylation in the sample.
Increase the SDS concentration in the
agarose overlay (up to 2%) and/or in
the SDS-PAGE running buffer (up to
0.4%). For incomplete reduction or
alkylation, increase the concentration of
reduction (DTT or TBP) or alkylation
(iodoacetamide) reagents, or use fresh
reagents.
4. Storing tube gels after IEF.
Extrude gel from the tube and store
(with or without buffer) at -20°C or
lower.
5. Further troubleshooting for
Refer to the Mini-PROTEAN cell
SDS-PAGE gels.
instruction manual.
Tygon is a trademark of Norton Co.
Parafilm is a trademark of American Can Company.
Intramedic is a trademark of Becton Dickinson & Co.
Triton is a trademark of Rohm and Haas, Co.
Eppendorf is a trademark of Eppendorf Geratebau Netheler + Hinz, GmbH.
SYPRO is a trademark of Molecular Probes.
14