Bio-Rad Model 225 Tube Gel Casting Stand - Manual (Page 5)

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Mini-PROTEAN tube cell (catalog # 165-2961). Includes tube cell module (1), sample reservoirs (2),
capillary tube connectors (3), tube setup; sample reservoirs/capillary tube connector/capillary tube (4),
capillary tubes (5), tube gel ejector (6), and casting tube (7).
2.2 Description of Major Parts
The Mini-PROTEAN 2-D cell (catalog number 165-2960) consists of the following
major parts:
1. Mini-PROTEAN electrophoresis cell for slab gels - A separate manual for the
Mini-PROTEAN cell is included. Refer to it for a complete description of the
slab cell parts.
2. Tube cell module - The tube cell module serves as the upper buffer chamber
and holds up to 16 tubes. The module comes with 16 plastic stoppers to plug
any tube holes not in use.
3. Sample reservoirs - The plastic sample reservoirs are molded with Luer
tapered ends which fit tightly into the 16 tube holes in the tube cell module. Gel
tubes are attached to the reservoirs with flexible Tygon tubing connectors.
4. Flexible tubing connectors (50) - These connectors are small pieces of Tygon
tubing about 7.5 mm long and 2 mm in outer diameter. They are used to connect
the sample reservoirs to the capillary gel tubes.
5. Capillary tubes (200) - The glass capillary tubes are used for running the first
dimension tube gels.
6. Casting tube - The casting tube is about 8.5 cm long and 1.3 cm in diameter. It
is used as a casting chamber to cast up to 40 capillary tube gels at once.
7. Tube gel ejector - The tube gel ejector is a green and white fitting which holds
the glass capillary tubes firmly at one end, without leaking, so that the gels can
be easily extruded. A series of O-rings in the fitting hold the tube tightly when
the gel ejector is used as described. The gel ejector must be used with a 1.0
ml syringe.
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