Bio-Rad Model 422 Electro-Eluter - Manual (Page 11)

Due to the absence of SDS in these buffer systems, expect the time required for elution to be
approximately 3-6 hours.
6.5 Removal of SDS from Samples Subsequent to Elution
SDS can be removed from the buffer and/or the sample in several ways:
1. Dialysis: Both electrodialysis, 2 and equilibrium dialysis, 2,3 have been used for SDS
removal. To electrodialyse, replace the lower buffer with fresh buffer made up without
SDS approximately half way through the run.
2. Triton X-100: This may be added to the elution buffer at approximately 0.5% concen-
tration. Triton can then be removed after the elution as per conventional methods. 4
3. AG ® 11 A8 ion retardation resin: Using this ion-retardation resin, an average of 83%
recovery of proteins may be expected, while 0.1 to 1.4 moles of SDS remain on each
mole of protein.
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4. Anion exchange separation: AG1-X2 anion exchange resin 6 has been employed for SDS
removal from proteins. Use of AG 1-X2 resin has resulted in 95% protein recovery with
at most only 1% of all protein molecules still binding 1 molecule of SDS.
5. Bio-Beads ® SM 2 beads. These macroporous support beads are mixed with the sample for
SDS removal.
1. Ornstein, L. and Davis, B. J., Ann N.Y., Acad. Sci., 121, 321 (1964).
2. Tuszynski, G. P., and Warren, L., Anal. Biochem., 67, 55 (1975).
3. Visser, L., and Blout, E. R., Biochem., 10, 743 (1971).
4. Holloway, P. W., Anal. Biochem., 53, 304 (1973).
5. Kapp, O. H., and Vinogradov, S. N., Anal. Biochem., 91, 230 (1978).
6. Weber, K., and Kuter, D. J., J. Biol.Chem., 246, 4504 (1971).
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