Bio-Rad Model 422 Electro-Eluter - Manual (Page 4)

Section 1
Introduction
Elution of proteins and nucleic acids from gel slices is often a difficult and unreliable
procedure. The Model 422 Electro-Eluter solves many of the problems of conventional eluters
by providing easy assembly and eliminating sample loss due to leakage. The Model 422
Electro-Eluter consistently yields between 70% and 100% of the starting material, depending
on the type of elution. The samples are collected in 400-600 µl volumes. The small volume
of eluted material makes subsequent manipulations easy. The Model 422 Electro-Eluter can
run up to six samples at once, with no increase in running time or decrease in yield.
1.1 Specifications
Construction
Electrode module Precision machined acrylic
Buffer chamber and lid Molded polycarbonate
Glass tubes Borosilicate glass
Frits Porous polypropylene
Silicone adaptors Molded silicone
Grommets and stoppers Molded silicone
Membrane caps Molded polyethylene with dialysis membrane
Overall size 16 cm (L) x 12 cm (W) x 18 cm (H)
Elution capacity 1 to 6 samples
Glass tube dimensions 1 cm ID x 6 cm long
Collection volume 400-600 µl
Buffer volume 700 ml
Shipping weight 1.1 kg
Chemical compatibility The Model 422 Electro-Eluter is not
compatible with chlorinated hydrocarbons
(e.g. chloroform), aromatic hydrocarbons
(e.g. toluene, benzene), or acetone. Use of
organic solvents voids all warranties.
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